Binding activity of replication protein A to single-stranded DNA containing oxidized pyrimidine base.

T Kasama, A Ono, S Izuta

Index: Nucleic Acids Res. Suppl. (1) , 167-8, (2001)

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Abstract

To obtain the information for the role of replication protein A (RPA) on the detection of oxidized lesion in the single-stranded DNA, the binding preference of RPA purified from Xenopus egg lysate against the oligonucleotide containing one of three kinds of oxidized thymine residues, 5-formyluracil, 5-hydroxymethyluracil and 5-(1,2-dihydroxyethyl)uracil, was studied by the gel shift assay. Results of competition assay indicate that RPA preferentially binds to the oligonucleotide containing these oxidized thymine residues than the undamaged DNA.