Plant Science 2015-09-01

Competency for shoot regeneration from Arabidopsis root explants is regulated by DNA methylation.

Or Shemer, Udi Landau, Héctor Candela, Assaf Zemach, Leor Eshed Williams

Index: Plant Sci. 238 , 251-61, (2015)

Full Text: HTML

Abstract

Plants exhibit high capacity to regenerate in three alternative pathways: tissue repair, somatic embryogenesis and de novo organogenesis. For most plants, de novo organ initiation can be easily achieved in tissue culture by exposing explants to auxin and/or cytokinin, yet the competence to regenerate varies among species and within tissues from the same plant. In Arabidopsis, root explants incubated directly on cytokinin-rich shoot inducing medium (SIM-direct), are incapable of regenerating shoots, and a pre-incubation step on auxin-rich callus inducing medium (CIM) is required to acquire competency to regenerate on the SIM. However the mechanism underlying competency acquisition still remains elusive. Here we show that the chromomethylase 3 (cmt3) mutant which exhibits significant reduction in CHG methylation, shows high capacity to regenerate on SIM-direct and that regeneration occurs via direct organogenesis. In WT, WUSCHEL (WUS) promoter, an essential gene for shoot formation, is highly methylated, and its expression on SIM requires pre-incubation on CIM. However, in cmt3, WUS expression induced by SIM-direct. We propose that pre-incubation on CIM is required for the re-activation of cell division. Following the transfer of roots to SIM, the intensive cell division activity continues, and in the presence of cytokinin leads to a dilution in DNA methylation that allows certain genes required for shoot regeneration to respond to SIM, thereby advancing shoot formation. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.


Related Compounds

Related Articles:

Neuropeptide Y in the noradrenergic neurones induces obesity and inhibits sympathetic tone in mice.

2015-04-01

[Acta Physiol. (Oxf.) 213(4) , 902-19, (2015)]

Targeting glucose uptake with siRNA-based nanomedicine for cancer therapy.

2015-05-01

[Biomaterials 51 , 1-11, (2015)]

Differential expression of efflux pump genes of Mycobacterium tuberculosis in response to varied subinhibitory concentrations of antituberculosis agents.

2015-03-01

[Tuberculosis (Edinb.) 95(2) , 155-61, (2015)]

Diclofenac toxicity in human intestine ex vivo is not related to the formation of intestinal metabolites.

2015-01-01

[Arch. Toxicol. 89(1) , 107-19, (2015)]

Effect of (2)H and (18)O water isotopes in kinesin-1 gliding assay.

2014-01-01

[PeerJ 2 , e284, (2014)]

More Articles...