Removal of bovine serum albumin using solid-phase extraction with in-situ polymerized stationary phase in a microfluidic device.

Eun Zoo Lee, Yun Suk Huh, Young-Si Jun, Hyo Jin Won, Yeon Ki Hong, Tae Jung Park, Sang Yup Lee, Won Hi Hong

Index: J. Chromatogr. A. 1187(1-2) , 11-7, (2008)

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Abstract

Serum albumin, one of the most abundant serum proteins, blocks the expression of other important biomarkers. The objective of this study is to remove serum albumin effectively by using solid-phase extraction (SPE) in microfluidic devices. Photo-polymerized adsorbent as a stationary phase of SPE was used to remove bovine serum albumin (BSA). The adsorption capacity was examined with the effect of pH and concentration in BSA solution, and adjustment of monomer concentration such as hydrophilic 2-acrylamido-2-methyl-1-propanesulfonic acid and acrylamide in the adsorbent. The effect of hydrophobic butyl methacylate on BSA adsorption was also studied. Selective removal in a bicomponent with BSA and bovine gamma-globulin was performed by adjusting the pH as required.