Biosynthesis of radiolabeled verruculogen by Penicillium simplicissimum.

J B Day, P G Mantle

Index: Appl. Environ. Microbiol. 43(3) , 514-6, (1982)

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Abstract

In surface culture of Penicillium simplicissimum, verruculogen was shown to be biosynthesized from the intact carbon skeletons of tryptophan and proline, isoprenoid derivatives of mevalonic acid, and a methyl group donated by methionine. Selected radiolabeled precursors (1 mCi) pulse-fed at the optimum stage of fermentation yielded verruculogen (specific activity, 5.89 X 10(2) microCi mmol-1) labeled in the prolyl and isoprenyl regions of the molecule and suitable for metabolic studies.