Analytical Biochemistry 1997-10-01

Spectrophotometric determination of the positional specificity of nonspecific and 1,3-specific lipases.

R N Farias, M Torres, R Canela

Index: Anal. Biochem. 252(1) , 186-9, (1997)

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Abstract

Using commercially available thiosubstrates, such as 2,3-dimercapto-1-propanol tributyrate, the regio-specificities of 1,3-specific and nonspecific lipases was confirmed. The spectrophotometric test is a simple, rapid, and convenient alternative method to those previously reported for the characterization of the positional specificities of new lipases.

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