Microarray analysis of juvenile hormone response in Drosophila melanogaster S2 cells.

David K Willis, Jun Wang, Joliene R Lindholm, Anthony Orth, Walter G Goodman

Index: J. Insect Sci. 10 , 66, (2010)

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Abstract

A microchip array encompassing probes for 14,010 genes of Drosophila melanogaster was used to analyze the effect of juvenile hormone (JH) on genome-wide gene expression. JH is a member of a group of insect hormones involved in regulating larval development and adult reproductive processes. Total RNA was isolated from Drosophila S2 cells after 4 hours treatment with 250 ng/ml (10R) JH III or 250 ng/ml methyl linoleate. A collection of 32 known or putative genes demonstrated a significant change with JH III treatment (r > 2.0, P