[Synthesis and identification of artificial antigens for sulfaguanidine and sulfathiazole].

Yanhua Cui, Hailing Zhang, Ying ma, Cuihong Dai, Jie Cui

Index: Wei Sheng Yan Jiu 39(4) , 440-3, (2010)

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Abstract

To prepare artificial antigens of sulfaguanidine and sulfathiazole for detecting sulfaguanidine and sulfathiazole in foods of animal origin.The artificial antigens were synthesized by the diazotization methods of coupling sulfaguanidine and sulfathiazole with bovine serum albumin (BSA), and the coupling ratio was 10 : 1 and 9 : 1 respectively. The anti-serum of Balb/c mice was obtained after 5 injections of artificial antigens and the antibody titles were detected by iELISA method.The artificial antigens were identified by infrared spectra, ultraviolet spectra and immunized experimental animals.The antigens for sulfaguanidine and sulfathiazole were acquired successfully. The results have laid basis for the specific immunoassay of sulfaguanidine and sulfathiazole.