Preparation of a stir bar coated with molecularly imprinted polymer and its application in analysis of dopamine in urine.

Yun Lei, Guanhong Xu, Fangdi Wei, Jing Yang, Qin Hu

Index: J. Pharm. Biomed. Anal. 94 , 118-24, (2014)

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Abstract

A molecularly imprinted polymer coated stir bar (MIP-SB) using dopamine as a template was fabricated. The adsorptive capacity of the MIP-SB was almost 4 times over that of non-imprinted stir bar. The MIP-SB showed good extracting selectivity for dopamine when used to adsorb dopamine and its analogs. An analytical method to determine dopamine in urine sample was established by combining MIP-SB sorptive extraction with HPLC-fluorescence detector. The linear range of dopamine concentration was 0.378-18.9ng/ml with correlation coefficient of 0.9990, and the limit of quantification was about 0.0945ng/ml (S/N=10). The recoveries of dopamine with spiked urine samples at three different levels were between 92.3 and 106.9%, and the relative standard deviations were within 13.2% (n=3). The method is simple and suitable for the determination of dopamine in human urine for clinical application. Copyright © 2014 Elsevier B.V. All rights reserved.