A peptide with potent antifungal and antiproliferative activities from Nepalese large red beans.
D Z Ma, H X Wang, T B Ng
Index: Peptides 30(12) , 2089-94, (2009)
Full Text: HTML
Abstract
An antifungal defensin-like peptide with a molecular mass of 7.1kDa was isolated from dried Nepalese large red beans (Phaseolus angularis). The purification protocol employed included ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-Sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. The antifungal peptide was unadsorbed on DEAE-cellulose, and adsorbed on Affi-gel blue gel and SP-Sepharose. The antifungal peptide inhibited mycelial growth in Fusarium oxysporum and Mycosphaerella arachidicola with an IC(50) value of 1.4 and 1.8 microM, respectively. It did not inhibit HIV-1 reverse transcriptase when tested up to 200 microM. It exerted an antiproliferative action on L1210 leukemia cells and MBL2 lymphoma cells with an IC(50) of 15 and 60 microM, respectively.
Related Compounds
Related Articles:
Ni speciation in tea infusions by monolithic chromatography--ICP-MS and Q-TOF-MS.
2013-02-01
[Anal. Bioanal. Chem 405(6) , 2041-51, (2013)]
Interaction of cellulose-based cationic polyelectrolytes with mucin.
2011-03-01
[Colloids Surf. B Biointerfaces 83(1) , 160-4, (2011)]
2007-05-01
[Comp. Biochem. Physiol. B Biochem. Mol. Biol. 147(1) , 56-73, (2007)]
2009-07-01
[Appl. Biochem. Biotechnol. 158(1) , 200-12, (2009)]
2011-01-01
[Int. J. Biol. Macromol. 48(1) , 38-43, (2011)]