Histochemistry 1989-01-01

Optimization of the histochemical demonstration of DNA using 3-hydroxy-2-naphthoic acid hydrazide and fast blue B.

G Nöhammer

Index: Histochemistry 90 , 465, (1989)

Full Text: HTML

Abstract

Previous methods for the histochemical demonstration of DNA were optimized. p-Toluene sulfonic acid as catalyst for hydrazone formation between the aldehydes generated after Feulgen hydrolysis and 3-hydroxy-2-naphthoic acid hydrazide (NAH) was used instead of acetic acid. Modifications of the conditions of the coupling reaction with Fast Blue B reduced the background staining. The optimized histochemical staining method for DNA (NAH-FB-DNA staining) can be performed easily and reproducibly. Without prior Feulgen hydrolysis the optimized method can also be used for the histochemical demonstration of reactive carbonyls undissolved under the given histochemical conditions.

Related Compounds

Related Articles:

Histochemical demonstration of protein-bound amino groups.

1954-01-01

[J. Histochem. Cytochem. 2(1) , 29-49, (1954)]

Synthesis of new 2,5-disubstituted-1,3,4-thiadiazoles and preliminary evaluation of anticonvulsant and antimicrobial activities.

2002-09-01

[Bioorg. Med. Chem. 10(9) , 2893-8, (2002)]

NADPH-dependent lipid peroxidation capacity in unfixed tissue sections: characterization of the pro-oxidizing conditions and optimization of the histochemical detection.

1994-03-01

[Histochem. J. 26(3) , 189-96, (1994)]

Imaging of oxidative stress at subcellular level by confocal laser scanning microscopy after fluorescent derivatization of cellular carbonyls.

1993-05-01

[Am. J. Pathol. 142(5) , 1353-7, (1993)]

The use of 3-hydroxy-2-naphthoic acid hydrazide and Fast Blue B for the histochemical detection of lipid peroxidation in animal tissues--a microphotometric study.

1991-01-01

[Histochemistry 95 , 255, (1991)]

More Articles...