[Effect on different concentrations of exogenous hormones on baicalin in Scutellaria baicalensis callus].

Gui-Xiang Wan, Lin Ma, Jian Zhang

Index: Zhongguo Zhong Yao Za Zhi 37(24) , 3799-802, (2012)

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Abstract

To determine the content of baicalin in Scutellaria baicalensis callus induced by different doncentrations of exogenous hormones.HPLC system was adopted to determine baicalin in S. baicalensis callus. Chromatographic conditions: ODS column was adopted, with methanol-water-phosphate (47: 53: 0.2) as the mobile phase. The flow velocity was 1 mL x min(-1), the detective wavelength was 280 nm, and the temperature of column was room temperature.S. baicalensis callus induced by 6-BA 1.0 mg x L(-1) + NAA 0.5 mg x L(-1) showed the highest baicalin content, up to 49.78 mg x g(-1).The experiment is such a simple, rapid and stable method for determining the baicalin content that it can be used for determining the baicalin content in S. baicalensis callus.