Evaluation of percutaneous absorption of esculetin: effect of chemical enhancers.

Sergio del Rio Sancho, César Eulogio Serna Jiménez, María Aracely Calatayud Pascual, Cristina Balaguer Fernández, Andrés Femenía Font, Encarna Castillo García, Virginia Merino, Alicia López Castellano

Index: Planta Med. 79(2) , 131-6, (2013)

Full Text: HTML

Abstract

Percutaneous transdermal absorption of esculetin (6,7-dihydroxycoumarin), an oxidative damage inhibitor, was evaluated by means of in vitro permeation studies in which vertical Franz-type diffusion cells and pig ear skin were employed. To determine the absorption of esculetin, we validated a simple, accurate, precise, and rapid HPLC-UV method. Additionally, the effects of several percutaneous enhancers were studied. Pretreatment of porcine skin was performed with ethanol (control vehicle), decenoic acid, oleic acid, R-(+)-limonene, and laurocapram (Azone®) (5% in ethanol, w/w, respectively). Pretreatment of skin with oleic acid or laurocapram led to statistically significant differences in the transdermal flux of esculetin with respect to controls. Of the two enhancers, laurocapram showed the greatest capacity to enhance the flux of esculetin across pig skin.Georg Thieme Verlag KG Stuttgart · New York.