Inhibition of aspartate aminotransferase by hydrazinosuccinate.

R H Yamada, Y Wakabayashi, A Iwashima, T Hasegawa

Index: Biochim. Biophys. Acta 801(1) , 151-4, (1984)

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Abstract

DL-Hydrazinosuccinic acid was synthesized by the reaction of DL-bromosuccinic acid with hydrazine. The compound strongly inhibited aspartate aminotransferase and gave 50% inhibition at 1.3 microM when added simultaneously with L-aspartate to an assay mixture containing enzyme. Incubation of the enzyme with the compound prior to assay resulted in a much stronger inhibition, which proceeded time-dependently. The inhibition was protectable with L-aspartate and was substantially reversed by dialysis.