Sensitive high-performance anion-exchange chromatographic determination of paeoniflorin and albiflorin by pulsed amperometric detection after solid-phase extraction.

Hee-Jung Sim, Ji-Seon Jeong, Ha-Jeong Kwon, Yong-Moon Lee, Seon-Pyo Hong

Index: J. Chromatogr. A. 1217(32) , 5302-5, (2010)

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Abstract

We developed a method to simultaneously determine paeoniflorin and albiflorin levels using high-performance anion-exchange liquid chromatography with pulsed amperometric detection (HPAEC-PAD). The main principle of our method includes solid-phase extraction step using Amberlite XAD-2 sorbent to remove sugars and to selectively determine glycosides by PAD. Under these conditions, the linear dynamic range was 0.01-100 microg/mL, and the albiflorin and paeoniflorin detection limits (S/N=3) were 5 and 10 pg, respectively. The intra- and inter-day precisions (RSDs) were <5.07%, and the average recoveries from Paeoniae Radix and Si-ni-san ranged from 97.12 to 101.15%. Our method showed high selectivity, high sensitivity, and good repeatability for analyzing albiflorin and paeoniflorin in oriental medicinal preparation.Copyright 2010 Elsevier B.V. All rights reserved.