[Determination of deoxyribonucleic acid with pararosaniline by a resonance light-scattering method].
Hai-yan Xiang, Xiao-ming Chen, Di-wu Zhou
Index: Guang Pu Xue Yu Guang Pu Fen Xi 22(6) , 1051-3, (2002)
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Abstract
The method is based on the enhancement effect of the resonance light-scattering (RLS) of pararosaniline by DNA. In the pH rang of 0.5-1.5, the enhanced intensity of pararosaniline RLS at 355 nm is proportional to the concentration of DNA in the rang of 0.20-15 micrograms.mL-1. The detection limit is 36 ng.mL-1. This method is simple, rapid and has been applied satisfactorily to the determination of DNA in mixed samples.
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