[Erythrocyte antibody diagnostic agents for determining the type and subtype of influenza viruses].

M Kh Saiatov, S E Asanova, R U Beĭsembaeva, B V Karal'nik

Index: Vopr. Virusol. 30(1) , 39-43, (1985)

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Abstract

The method of antibody sensitization of sheep erythrocyte using amidole was employed to prepare diagnosticums for influenza A/USSR/090/77 (H1N1), A/Bangkok/1/79 (H3N2), and B/Hong Kong/75 viruses. Comparison of the potency of diagnosticums obtained on the basis of hyperimmune influenza sera and immunoglobulin preparations demonstrated that the use of globulins isolated with ammonium sulphate, by gel filtration on Sephadex G-200, and ion-exchange chromatography on DEAE-cellulose 32 gives a higher sensitivity of the HI test than that obtained with sera and Fab-2 fragments of antibodies. The diagnosticums produced by the amidole method show higher specificity and facilitate the determination of the type and subtype appurtenance of epidemic and inter-epidemic influenza virus strains. In determinations of hemagglutinin the sensitivity of HI test with subtype homologous antibody diagnosticum was 2-8 times higher than that of HA test.