Biotechnology Progress 2012-01-01

Biotransformation of 2,6-diaminopurine nucleosides by immobilized Geobacillus stearothermophilus.

Eliana C De Benedetti, Cintia W Rivero, Claudia N Britos, Mario E Lozano, Jorge A Trelles

Index: Biotechnol. Prog. 28(5) , 1251-6, (2012)

Full Text: HTML

Abstract

An efficient and green bioprocess to obtain 2,6-diaminopurine nucleosides using thermophilic bacteria is herein reported. Geobacillus stearothermophilus CECT 43 showed a conversion rate of 90 and 83% at 2 h to obtain 2,6-diaminopurine-2'-deoxyriboside and 2,6-diaminopurine riboside, respectively. The selected biocatalyst was successfully stabilized in an agarose matrix and used to produce up to 23.4 g of 2,6-diaminopurine-2'-deoxyriboside in 240 h of process. These nucleoside analogues can be used as prodrug precursors or in antisense oligonucleotide synthesis.Copyright © 2012 American Institute of Chemical Engineers (AIChE).

Related Compounds

Related Articles:

Site-specific fluorescence dynamics in an RNA 'thermometer' reveals the role of ribosome binding in its temperature-sensitive switch function.

2015-01-01

[Nucleic Acids Res. 43(1) , 493-503, (2015)]

Pro-inflammatory cytokine/chemokine production by reovirus treated melanoma cells is PKR/NF-κB mediated and supports innate and adaptive anti-tumour immune priming.

2011-01-01

[Mol. Cancer 10 , 20, (2011)]

Ligand-induced evolution of intrinsic fluorescence and catalytic activity from cobalt ferrite nanoparticles.

2015-06-08

[ChemPhysChem 16 , 1627-34, (2015)]

CGP74514A enhances TRAIL-induced apoptosis in breast cancer cells by reducing X-linked inhibitor of apoptosis protein.

2014-07-01

[Anticancer Res. 34(7) , 3557-62, (2014)]

Synthesis of 9,9'-[1,2-ethanediylbis(oxymethylene)]bis-2-amino-1,9-dihydro-6H-purin-6-one, an impurity of acyclovir.

2012-01-01

[Molecules 17(8) , 8735-41, (2012)]

More Articles...