Electrochemiluminescence DNA sensor array for multiplex detection of biowarfare agents.

Anna-Maria Spehar-Délèze, Rainer Gransee, Sergio Martinez-Montequin, Diego Bejarano-Nosas, Samuel Dulay, Sandra Julich, Herbert Tomaso, Ciara K O'Sullivan

Index: Anal. Bioanal. Chem 407 , 6657-67, (2015)

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Abstract

Development of a fully automated electrochemiluminescence (ECL) DNA assay for multiplex detection of six biowarfare agents is described. Aminated-DNA capture probes were covalently immobilised on activated-carbon electrodes and subsequently hybridised to target strands. Detection was achieved via a sandwich-type assay after Ru(bpy)3(2+)-labelled reporter probes were hybridised to the formed probe-target complexes. The assay was performed in an automated microsystem in a custom designed ECL detection box with integrated fluidics, electronics,and movable photomultiplier detector. The obtained limits of detection were 0.6-1.2 nmol L(-1) for six targets ranging from 50 to 122 base pairs in size, with linear range 1-15 nmol L(-1). Non-specific adsorption and cross-reactivity were very low. Detection of six targets on a single chip was achieved with subnanomolar detection limits.