Ionic liquid-reconstituted cellulose composites as solid support matrices for biocatalyst immobilization.

Megan B Turner, Scott K Spear, John D Holbrey, Daniel T Daly, Robin D Rogers

Index: Biomacromolecules 6(5) , 2497-502, (2005)

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Abstract

Preparation of cellulose-polyamine composite films and beads, which provide high loading of primary amines on the surface allowing direct one-step bioconjugation of active species, is reported using an ionic liquid (IL) dissolution and regeneration process. Films and bead architectures were prepared and used as immobilization supports for laccase as a model system demonstrating the applicability of this approach. Performance of these materials, compared to commercially available products, has been assessed using millimeter-sized beads of the composites and the lipase-catalyzed transesterification of ethyl butyrate.