Simultaneous determination of malachite green, gentian violet and their leuco-metabolites in aquatic products by high-performance liquid chromatography-linear ion trap mass spectrometry.

Xueli Wu, Gong Zhang, Yongning Wu, Xiaolin Hou, Zonghui Yuan

Index: J. Chromatogr. A. 1172(2) , 121-6, (2007)

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Abstract

A method has been developed for the simultaneous determination of malachite green, gentian violet and their leuco-metabolites in various aquatic products using isotope dilution liquid chromatography-linear ion trap mass spectrometry without post-column oxidation. Sample was extracted with McIlvaine buffer and acetonitrile, followed by partitioning with dichloromethane, purified on basic alumina and OASIS MCX SPE column, and finally analyzed by LC-ESI-MS/MS with the select reaction monitoring (SRM) mode. Decision limits (CCalpha, alpha=0.01) and detection capability (CCbeta, beta=0.05) of the method were in the range of 0.02-0.09 and 0.04-0.13microg/kg for MG, GV, LMG and LGV in grass carp, eel, salmon, shrimp and shellfish, respectively, recoveries of MG, GV, LMG and LGV at all fortification levels (0.25-10microg/kg) were from 80.8% to 115.7%, inter-day relative standard derivations were from 1.9% to 18.4%. This method appeared suitable for the control of MG, GV, LMG and LGV residues in aquatic products.