A catalytic antibody against a tocopherol cyclase inhibitor

R Manetsch, L Zheng, MT Reymond…

Index: Manetsch, Roman; Zheng, Lei; Reymond, Martine T.; Woggon, Wolf-Dietrich; Reymond, Jean-Louis Chemistry - A European Journal, 2004 , vol. 10, # 10 p. 2487 - 2506

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Citation Number: 22

Abstract

Abstract The cyclic ammonium cation 5 and its guanidinium analogue 4 are inhibitors of tocopherol cyclase. Monoclonal antibodies were raised against protein conjugates of the haptens 1–3 and screened for catalytic reactions with alkene 8, a short chain analogue of the natural substrate phytyl-hydroquinone 6, and its enol ether analogues 10 a, b. Antibody 16E7 raised against hapten 3 was found to catalyze the hydrolysis of Z enol ether 10 a to ...