Preparation of peptide and other biomolecular conjugates through chemoselective ligations.

Mathieu Galibert, Olivier Renaudet, Didier Boturyn, Pascal Dumy

Index: Methods Mol. Biol. 751 , 67-79, (2011)

Full Text: HTML

Abstract

The synthesis of molecular conjugates through chemoselective ligations represents a very convenient strategy to prepare complex macromolecules with diverse functional elements. Herein, we describe chemical methods based on the preparation of chemoselectively addressable peptides allowing successive oxime ligations and/or alkyne-azide cycloaddition ("click") reactions of various biomolecules. This modular synthetic approach can be applied to a broad range of purposes.