[Effect of osthole on bone metabolism in rat femoral tissues in vitro].

Jian Zhou, Bao-feng Ge, Ping Zhen, Xiao-ni Ma, Li-juan Yan, Xiao-Yu Guo, Kui Cheng, Yu-Hai Gao, Wen-Gui Shi, Ke-ming Chen

Index: Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 35(5) , 561-6, (2013)

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Abstract

To investigate the effect of osthole on bone metabolism in rat femoral tissues in vitro.The rat femoral tissues were isolated in vitro. The optimal concentrations of ostehole (1×10(-5) mol/L) and estradiol (1×10(-8) mol/L) (the positive control) were selected by alkaline phosphatase activity (ALP). The ALP and calcium levels were detected by commmerical regents, and the expressions of osteoprotegerin, receptor activator of nuclear factor-κB ligand, runx-related gene 2, and bone morphogenetic protein-2 mRNA were determined by real-time reverse transcription-polymerase chain reaction.The osthole (1×10(-5) mol/L) significantly increased the activity of ALP, calcium level as well as the expressions of osteoprotegerin, receptor activator of nuclear factor-κB ligand, runx-related gene-2 and bone morphogenetic protein-2 mRNA in rat femoral tissues in vitro.Osthole can improve calcium level and ALP activity and regulate the bone metabolism-related genes in rat femoral tissues.