Electrochemical evaluation of poly(3,4-ethylenedioxythiophene) films doped with bacteria based on viability analysis.

Dung Quynh Le, Shiho Tokonami, Tomoaki Nishino, Hiroshi Shiigi, Tsutomu Nagaoka

Index: Bioelectrochemistry 105 , 50-5, (2015)

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Abstract

To immobilize viable bacteria on an electrode, we present a novel and straightforward technique that relies on the negative ζ-potentials of bacteria for insertion into conducting polymers as dopants. In the present study, we conducted an electrochemical polymerization of poly(3,4-ethylenedioxythiophene) (PEDOT) doped with various gram-negative bacteria, including Pseudomonas aeruginosa, Escherichia coli, and Shewanella oneidensis. The PEDOT film doped with bacteria indicated a typical redox response, high conductivity, and electrochemical stability. Fluorescence microscopy confirmed that approximately 90% of the bacteria incorporated into the PEDOT film at >0.5 μm in thickness were viable.Copyright © 2015 Elsevier B.V. All rights reserved.