Structural features and antioxidant activity of xylooligosaccharides enzymatically produced from sugarcane bagasse.

Jing Bian, Feng Peng, Xiao-Peng Peng, Pai Peng, Feng Xu, Run-Cang Sun

Index: Bioresour. Technol. 127 , 236-41, (2013)

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Abstract

Xylooligosaccharides (XOS) were prepared from xylan-rich hemicelluloses isolated by potassium hydroxide from sugarcane bagasse by hydrolysis with crude xylanase secreted by Pichia stipitis. Hydrolysis for 12h produced XOS with a maximum yield of 31.8%, equivalent to 5.29 mg mL(-1) in the hydrolyzate. XOS with degrees of polymerization (DP) from 2 to 4 (xylobiose, xylotriose, and xylotetraose) were the major components in the hydrolysates, whereas the oligosaccharides with higher DP of 5-6 (xylopentaose and xylohexose) showed a constant low level. FT-IR and NMR ((1)H, (13)C, HSQC) demonstrated that XOS contained Araf and 4-O-Me-α-D-GlcpA residues. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay showed that the XOS exhibited concentration-dependent antioxidant activity. The results obtained indicate that the XOS produced from sugarcane bagasse can be employed in food-related applications.Copyright © 2012 Elsevier Ltd. All rights reserved.