Synthesis of 3′-O-(2-cyanoethyl)-2′-deoxythymidine-5′-phosphate as a model compound for evaluation of cyanoethyl cleavage

…, DC Knapp, M Kwiatkowski, JW Engels

Index: Keller, Angelika C.; Serva, Saulius; Knapp, Diana C.; Kwiatkowski, Marek; Engels, Joachim W. Collection of Czechoslovak Chemical Communications, 2009 , vol. 74, # 4 p. 515 - 534

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Citation Number: 7

Abstract

Abstract An essential and challenging task during the development of our sequencing-by- synthesis (SBS) technique is the evaluation of efficient cyanoethyl (CE) cleavage conditions. For this purpose 3′-O-(2-cyanoethyl)-2′-deoxythymidine-5′-phosphate as a model compound as well as a short DNA oligomer bearing the CE function as terminal group were synthesized and used for various deprotection experiments. As it is already known for 2′- ...