Polynomial algebra reveals diverging roles of the unfolded protein response in endothelial cells during ischemia-reperfusion injury.

Sylvain Le Pape, Elena Dimitrova, Patrick Hannaert, Alexander Konovalov, Romain Volmer, David Ron, Raphaël Thuillier, Thierry Hauet

Index: FEBS Lett. 588(17) , 3062-7, (2014)

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Abstract

The unfolded protein response (UPR)--the endoplasmic reticulum stress response--is found in various pathologies including ischemia-reperfusion injury (IRI). However, its role during IRI is still unclear. Here, by combining two different bioinformatical methods--a method based on ordinary differential equations (Time Series Network Inference) and an algebraic method (probabilistic polynomial dynamical systems)--we identified the IRE1α-XBP1 and the ATF6 pathways as the main UPR effectors involved in cell's adaptation to IRI. We validated these findings experimentally by assessing the impact of their knock-out and knock-down on cell survival during IRI.Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.