Phytotherapy Research 2012-11-01

Oleuropein, chief constituent of olive leaf extract, prevents the development of morphine antinociceptive tolerance through inhibition of morphine-induced L-type calcium channel overexpression.

Leila Zare, Saeed Esmaeili-Mahani, Mehdi Abbasnejad, Bahram Rasoulian, Vahid Sheibani, Hedayat Sahraei, Ayat Kaeidi

Index: Phytother Res. 26(11) , 1731-7, (2012)

Full Text: HTML

Abstract

It has been shown that blockade of L-type calcium channels could abolish the development of opioid-induced antinociceptive tolerance. Here, the antitolerant effects of olive leaf extract (OLE) and its main component, oleuropein, which have a calcium channel blocker property were determined. Adult male Wistar rats were injected with morphine (20 mg/kg, i.p.) for 8 days to induce antinociceptive tolerance. Then OLE (50-200 mg/kg i.g.) and oleuropein (1-10 mg/kg i.p.) were injected concomitantly with morphine. The tail-flick test was used to assess the nociceptive threshold. The dorsal half of the lumbar spinal cord was assayed for the expression of L-type calcium channel using semiquantitative RT-PCR. The results showed that OLE (200 mg/kg) completely prevented morphine tolerance development. In addition, oleuropein in dose of 10 mg/kg, but not in 5 mg/kg, prevented the development of morphine antinociceptive tolerance. In addition, a significant increase in the mRNA levels of calcium channel (43.9%) was observed in the lumbar spinal cord of tolerant animals, which was reversed by effective of dose OLE. In conclusion, the results indicate that olive leaf extract has a potential antitolerant property against the chronic usage of morphine and that its main component, oleuropein, is responsible for such effect.Copyright © 2012 John Wiley & Sons, Ltd.


Related Compounds

Related Articles:

Rapid screening of oleuropein from olive leaves using matrix solid-phase dispersion and high-performance liquid chromatography.

2014-01-01

[J. AOAC Int. 97(4) , 1109-13, (2014)]

A metabolite-profiling approach to assess the uptake and metabolism of phenolic compounds from olive leaves in SKBR3 cells by HPLC-ESI-QTOF-MS.

2013-01-01

[J. Pharm. Biomed. Anal. 72 , 121-6, (2013)]

Antiproliferative effect of oleuropein in prostate cell lines.

2012-07-01

[Int. J. Oncol. 41(1) , 31-8, (2012)]

Application of nanoLC-ESI-TOF-MS for the metabolomic analysis of phenolic compounds from extra-virgin olive oil in treated colon-cancer cells.

2012-04-07

[J. Pharm. Biomed. Anal. 63 , 128-34, (2012)]

Characterisation and phenolic profiles of two rare olive oils from southern Tunisia: Dhokar and Gemri-Dhokar cultivars.

2013-02-01

[J. Sci. Food Agric. 93(3) , 527-34, (2013)]

More Articles...