Determination of benzydamine and its N-oxide in biological fluids by high-performance liquid chromatography.

G A Baldock, R R Brodie, L F Chasseaud, T Taylor

Index: J. Chromatogr. A. 529(1) , 113-23, (1990)

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Abstract

A simple, sensitive and selective method for the determination of benzydamine in human plasma and urine, and for benzydamine N-oxide in urine, has been developed using high-performance liquid chromatography in the reversed-phase mode. The limit of reliable determination of benzydamine in plasma was 0.5 ng/ml and that in urine 1 ng/ml; the limit of reliable determination of benzydamine N-oxide in urine was 50 ng/ml. The method has been successfully applied to the analysis of these compounds in biological fluids after administration of intravenous and oral doses of benzydamine to human volunteers.