Biocompatible chiral monolithic stationary phase synthesized via atom transfer radical polymerization for high performance liquid chromatographic analysis.

Huai-Song Wang, Xia-Yi Feng, Ji-Ping Wei

Index: J. Chromatogr. A. 1409 , 132-7, (2015)

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Abstract

Novel biocompatible chiral monolithic stationary phase was prepared by reverse and direct atom transfer radical polymerization (ATRP) methods. By taking advantages of the controlled/living property of ATRP method, the chiral monolith was prepared by reverse ATRP (RATRP) firstly. An attractive feature of RATRP is the prepared polymer containing a terminal radically transferable atom that can initiate another post-polymerization reaction by direct ATRP. Then, the biocompatible poly(hydroxyethyl methacrylate) (PHEMA) was grafted on the surface of the chiral monolith by direct ATRP as a diffusion barrier for proteins. This biocompatible chiral monolith was successfully used as restricted access stationary phase for determination of enantiomers in biological samples with direct injection by high-performance liquid chromatography (HPLC). Copyright © 2015 Elsevier B.V. All rights reserved.