Genotoxicity of Superparamagnetic Iron Oxide Nanoparticles in Granulosa Cells.

Marina Pöttler, Andreas Staicu, Jan Zaloga, Harald Unterweger, Bianca Weigel, Eveline Schreiber, Simone Hofmann, Irmi Wiest, Udo Jeschke, Christoph Alexiou, Christina Janko

Index: Int. J. Mol. Sci. 16 , 26280-90, (2015)

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Abstract

Nanoparticles that are aimed at targeting cancer cells, but sparing healthy tissue provide an attractive platform of implementation for hyperthermia or as carriers of chemotherapeutics. According to the literature, diverse effects of nanoparticles relating to mammalian reproductive tissue are described. To address the impact of nanoparticles on cyto- and genotoxicity concerning the reproductive system, we examined the effect of superparamagnetic iron oxide nanoparticles (SPIONs) on granulosa cells, which are very important for ovarian function and female fertility. Human granulosa cells (HLG-5) were treated with SPIONs, either coated with lauric acid (SEONLA) only, or additionally with a protein corona of bovine serum albumin (BSA; SEON(LA-BSA)), or with dextran (SEON(DEX)). Both micronuclei testing and the detection of γH2A.X revealed no genotoxic effects of SEON(LA-BSA), SEON(DEX) or SEON(LA). Thus, it was demonstrated that different coatings of SPIONs improve biocompatibility, especially in terms of genotoxicity towards cells of the reproductive system.