Engineering of yeast pyruvate decarboxylase for enhanced selectivity towards carboligation.

Praveen Kumar Agarwal, Vanita Uppada, A G Swaminathan, Santosh B Noronha

Index: Bioresour. Technol. 192 , 90-6, (2015)

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Abstract

The aim of the study was to increase production of (R)-PAC by altering carboligation activity of Pdc in Saccharomyces cerevisiae. Pdc1 activity was modified by over-expression as well as changing the rate of decarboxylation and carboligation by site specific mutation in Pdc1. Over-expression of mutant Pdc1 resulted in 50 ± 2.5% increase in levels of (R)-PAC in wild-type and further 30-40% in pdc null background. The combination of mutant Pdc1 in pdc null background was successfully evaluated for production of (R)-PAC at industrial scale. This is the first report of enhancing (R)-PAC product in yeast by recombinant technology with capability of commercial production.Copyright © 2015 Elsevier Ltd. All rights reserved.