Binding mechanisms for histamine and agmatine ligands in plasmid deoxyribonucleic acid purifications

Ângela Sousa, Patrícia Pereira, Fani Sousa, João A. Queiroz

Index: J. Chromatogr. A. 1366 , 110-9, (2014)

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Abstract

• The versatility of histamine and agmatine ligands in function of binding and elution conditions. • Histamine and agmatine-pDNA multiple interactions depend of salts, ionic strength and pH. • Both ligands showed a biorecognition to the sc pDNA isoform. • The binding and elution conditions affect the pDNA structural stability.