Layer by layer assembly of biotinylated protein networks for signal amplification.

Yu W Chu, Bo Y Wang, Huei-Shian Lin, Tai-Yen Lin, Yuan-Jen Hung, David A Engebretson, Wonjae Lee, James R Carey

Index: Chem. Commun. (Camb.) 49(24) , 2397-9, (2013)

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Abstract

Described herein is a unique and inexpensive method that outperforms commercial methods that amplify the streptavidin-biotin recognition event. Amplification induced by streptavidin and biotinylated protein causes the formation of a large detectable polymer. This approach enjoys a 100-fold decrease in detection limit in comparison with the commercial methods.