Near-infrared fluorescence probes for enzymes based on binding affinity modulation of squarylium dye scaffold

D Oushiki, H Kojima, Y Takahashi, T Komatsu…

Index: Oushiki, Daihi; Kojima, Hirotatsu; Terai, Takuya; Arita, Makoto; Hanaoka, Kenjiro; Urano, Yasuteru; Nagano, Tetsuo Journal of the American Chemical Society, 2010 , vol. 132, # 8 p. 2795 - 2801

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Citation Number: 16

Abstract

We present a novel design strategy for near-infrared (NIR) fluorescence probes utilizing dye– protein interaction as a trigger for fluorescence enhancement. The design principle involves modification of a polymethine dye with cleavable functional groups that reduce the dye's protein-binding affinity. When these functional groups are removed by specific interaction with the target enzymes, the dye's protein affinity is restored, protein binding occurs, and ...