Pegylation effect of chitosan based polyplex on DNA transfection.
Wen Jen Lin, Wan Yi Hsu
Index: Carbohydr. Polym. 120 , 7-14, (2015)
Full Text: HTML
Abstract
The aim of this study was to develop hepatocyte-targeting non-viral polymeric nono-carriers for gene delivery. Chitosan was selected as the main polymer. An asialoglycoprotein receptor recognized sugar, galactose, was introduced. The methoxy poly(ethylene glycol) (mPEG) or short chain poly(ethylene glycol) diacid (PEGd) was further grafted onto galactosylated chitosan. All polyplex possessed positive charge character. The compaction of DNA by grafted chitosan was in order of chitosan-galactose-mPEG>chitosan-galactose-PEGd>chitosan-galactose where the chitosan-galactose-mPEG and pDNA formed the most stable polyplex. The polyplex prominently enhanced DNA cellular transfection as compared to naked DNA in HepG2 cells in order of chitosan-galactose/pDNA (11.6±0.6-33.0±4.4%)>chitosan-galactose-PEGd/pDNA (12.7±2.5-15.5±3.0%)>chitosan-galactose-mPEG/pDNA (9.0±1.1-12.9±2.4%). Copyright © 2014 The Authors. Published by Elsevier Ltd.. All rights reserved.
Related Compounds
Related Articles:
Aptamer-based polyvalent ligands for regulated cell attachment on the hydrogel surface.
2015-04-13
[Biomacromolecules 16(4) , 1382-9, (2015)]
2014-10-01
[J. Comp. Physiol. B, Biochem. Syst. Environ. Physiol. 184(7) , 865-76, (2014)]
Polymerization of affinity ligands on a surface for enhanced ligand display and cell binding.
2014-12-08
[Biomacromolecules 15(12) , 4561-9, (2014)]
2014-08-07
[Nanoscale 6(15) , 8720-5, (2014)]
Effect of (2)H and (18)O water isotopes in kinesin-1 gliding assay.
2014-01-01
[PeerJ 2 , e284, (2014)]