Fabrication of graphene-platinum nanocomposite for the direct electrochemistry and electrocatalysis of myoglobin.

Wei Sun, Linfang Li, Bingxin Lei, Tongtong Li, Xiaomei Ju, Xiuzheng Wang, Guangjiu Li, Zhenfan Sun

Index: Mater. Sci. Eng. C. Mater. Biol. Appl. 33(4) , 1907-13, (2013)

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Abstract

In this paper a platinum (Pt) nanoparticle decorated graphene (GR) nanosheet was synthesized and used for the investigation on direct electrochemistry of myoglobin (Mb). By integrating GR-Pt nanocomposite with Mb on the surface of carbon ionic liquid electrode (CILE), a new electrochemical biosensor was fabricated. UV-Vis absorption and FT-IR spectra indicated that Mb remained its native structure in the nanocomposite film. Electrochemical behaviors of Nafion/Mb-GR-Pt/CILE were investigated with a pair of well-defined redox peak appeared, which indicated that direct electron transfer of Mb was realized on the underlying electrode with the usage of the GR-Pt nanocomposite. The fabricated electrode showed good electrocatalytic activity to the reduction of trichloroacetic acid in the linear range from 0.9 to 9.0 mmol/L with the detection limit as 0.32 mmol/L (3σ), which showed potential application for fabricating novel electrochemical biosensors and bioelectronic devices.Copyright © 2012 Elsevier B.V. All rights reserved.