Structural Characterization of Neutral Glycosphingolipids from 3T3-L1 Adipocytes.

Hisao Kojima, Yusuke Suzuki, Masahiro Ito, Kazuya Kabayama

Index: Lipids 50 , 913-7, (2015)

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Abstract

In recent years, obesity has been considered a pathological stage of early lifestyle-related diseases, and adipose tissue and adipocyte research has been active. Glycosphingolipids are involved in the pathogenesis of type 2 diabetes induced by insulin resistance, but the details of the glycosphingolipid molecular species composition of adipocytes have yet to be elucidated. We used 3T3-L1 adipocytes and the 1,2-dichloroethane-wash method to remove triacylglycerols, which are abundant in adipocytes, and analyzed the structures of glycosphingolipids, particularly neutral glycosphingolipids, using liquid chromatography-mass spectrometry.