Formulation of highly functionalizable DNA nanoparticles based on 1,2-dithiolane derivatives.

Coralie Charrat, Anaïs Biscotti, Guilhem Godeau, Jacques Greiner, Pierre Vierling, Jean-Marie Guigonis, Christophe Di Giorgio

Index: ChemBioChem. 16(5) , 792-804, (2015)

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Abstract

We describe the formulation of synthetic virus models based on ionic compounds bearing the polymerizable 1,2-dithiolane moiety. First, cationic amphiphiles containing the polymeric inducer were prepared and used to efficiently condense a DNA plasmid (pDNA) into a highly monodisperse population of small polymeric cationic DNA nanoparticles (NPs; Dh ∼100 nm). These nonspecific cationic particles were then functionalized with anionic PEGylated conjugates, also based on the 1,2-dithiolane motifs, in order to produce stable and fully dispersible stealth DNA nanoparticles. Our results show that both ionic interactions and polymerization based on the 1,2-dithiolane pattern occur and that they produce highly functionalizable nonviral DNA NPs. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.