A new route for synthesis of silver:gold alloy nanoparticles loaded within phosphatidylcholine liposome structure as an effective antibacterial agent against Pseudomonas aeruginosa.

Amir H Salehi, Majid Montazer, Tayebeh Toliyat, Mahnaz Mahmoudi-Rad

Index: J. Liposome Res. 25(1) , 38-45, (2015)

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Abstract

Ag:Au alloy nanoparticles were successfully synthesized through the new route using co-reduction method with silver nitrate, chloroauric acid, cetyl trimethyl ammonium bromide (CTAB) and sodium borohydride at room temperature. The Ag:Au alloy nanoparticles were then loaded within the phosphatidylcholine (97%) liposome structure. Various molar ratios of phosphotidylcholine and CTAB to the total metals were investigated showing its importance on the stability of nanocomposites suspension. The size distribution and morphology of encapsulated nanoparticles within the liposome structure were studied via ultraviolet (UV)-visible spectrum, transmission electron microscope (TEM) micrographs, and dynamic light scattering data. The synthesis of alloy nanoparticles were confirmed with formation of single band at 430, 465 and 500 nm for 75:25, 50:50 and 25:75 Ag:Au mole ratios, respectively. The TEM micrographs of different samples indicated formation of three various nanocomposite structures with size of 82-300 nm. The antibacterial activities of Ag:Au nanocomposites were studied against Pseudomonas aeruginosa through well-diffusion agar. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by Broth microdilution method. The results showed that 10 ppm nanocomposite reasonably killed the above bacteria.