Isolation and identification of diadenosine 5′, 5‴-P 1, P 4-tetraphosphate binding proteins using magnetic bio-panning

…, MA Azhar, Y Xu, M Wright, A Kamal, AD Miller

Index: Guo, Wei; Azhar, M. Ameruddin; Xu, Yuhong; Wright, Michael; Kamal, Ahmed; Miller, Andrew D. Bioorganic and Medicinal Chemistry Letters, 2011 , vol. 21, # 23 p. 7175 - 7179

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Citation Number: 5

Abstract

We report the development of a synthetic, biotin-conjugated diadenosine tetraphosphate (Ap4A)-'molecular hook'attached to magnetic beads enabling the isolation of Ap4A-binding proteins from bacterial cells or mammalian tissue lysates. Characterisation and identification of isolated binding proteins is performed sequentially by mass spectrometry. The observation of positive controls suggests that these newly observed proteins are putative ...