Preparation of chlorophylls and pheophytins by isocratic liquid chromatography

T Watanabe, A Hongu, K Honda, M Nakazato…

Index: Watanabe, Tadashi; Hongu, Akinori; Honda, Kenichi; Nakazato, Masataka; Konno, Mitsuo; Saitoh, Sadao Analytical Chemistry, 1984 , vol. 56, # 2 p. 251 - 256

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Citation Number: 176

Abstract

Isocratlc high-performance liquid chromatography (HPLC) wlth silica gel as a stationary phase provides a powerful means for rapld preparative Isolation (on a 20-50 mg level) of chlorophylls (a, a', b, and b') and pheophytlns (a, a', b, and b'). The purity and Identity of the Isolated pigments have been conflrrned by complete elemental analyses and analytlcal HPLC; the purity levels were 99.9, 99.5, 99.5, 99.4, 95, 91, 99.5, and 85% for chlorophyll a ...