Stabilization of anionic and neutral forms of a fluorophoric ligand at the active site of human carbonic anhydrase I

S Manokaran, J Banerjee, S Mallik…

Index: Manokaran, Sumathra; Banerjee, Jayati; Mallik, Sanku; Srivastava Biochimica et Biophysica Acta - Proteins and Proteomics, 2010 , vol. 1804, # 10 p. 1965 - 1973

Full Text: HTML

Citation Number: 0

Abstract

We synthesized a fluorogenic dansylamide derivative (JB2-48), which fills the entire (15Å deep) active site pocket of human carbonic anhydrase I, and investigated the contributions of sulfonamide and hydrophobic regions of the ligand structure on the spectral, kinetic, and thermodynamic properties of the enzyme–ligand complex. The steady-state and fluorescence lifetime data revealed that the deprotonation of the sulfonamide moiety of the ...