How Does Lipase Flexibility Affect Its Enantioselectivity in Organic Solvents? A Possible Role of CH.... PI. Association in Stabilization of Enzyme-Substrate Complex

K Watanabe, T Uno, T Koshiba, T Okamoto…

Index: Watanabe, Keiichi; Uno, Tetsuya; Koshiba, Takashi; Okamoto, Takashi; Ebara, Yasuhito; Ueji, Shin-Ichi Bulletin of the Chemical Society of Japan, 2004 , vol. 77, # 3 p. 543 - 548

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Citation Number: 7

Abstract

For lipase-catalyzed reactions of 2-(4-substitued phenoxy) propionic acids with alcohols in organic solvents containing a small amount of water, the increase of the lipase flexibility brought about by addition of water is found to be favorable for the induced-fit motion of the lipase for the correctly binding enantiomer of the substrate used, thus resulting in the improvement of the lipase enantioselectivity. In particular, for the reaction of the substrate ...