Three Novel Spin??Labeled Substrates for Enzymatic Incorporation into Nucleic Acid Lattices

CR Toppin, GT Pauly, P Devanesan…

Index: Toppin, Charles R.; Pauly, Gary T.; Devanesan, Prabu; Kryak, David; Bobst, Albert M. Helvetica Chimica Acta, 1986 , vol. 69, p. 345 - 349

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Citation Number: 8

Abstract

Abstract The synthesis of one C (4)-spin-labeled uridine-5′-diphosphate (C (4)-UDP) and two nitroxide-containing 2′-deoxyuridine-5′-triphosphate (dUTP) analogs are reported. The C (4)-UDP derivative was incorporated into copolymers by polynucleotide phosphoxylase (PNPase); one of the two dUTP analogs, substituted at position C (4), was a good substrate for TdT, whereas the other one, substituted at position C (5), served as ...