Fingerprint lipolytic enzymes with chromogenic p-nitrophenyl esters of structurally diverse carboxylic acids

L Qian, JY Liu, JY Liu, HL Yu, CX Li, JH Xu

Index: Qian, Le; Liu, Jia-Yan; Liu, Jia-Ying; Yu, Hui-Lei; Li, Chun-Xiu; Xu, Jian-He Journal of Molecular Catalysis B: Enzymatic, 2011 , vol. 73, # 1-4 p. 22 - 26

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Citation Number: 11

Abstract

A series of structurally diverse chromogenic esters, including a new compound (4- nitrophenyl 2-methylpentanoate), has been synthesized, constituting an array of 17 substrates which could be applied to rapidly fingerprint the activity of lipases or esterases to reveal their substrates specificity and functional characteristics. Combined with genetic technology such as “data mining” and directed evolution, such fingerprints might be a ...