Chemistry & biology

Useful tools for biomolecule isolation, detection, and identification: acylhydrazone-based cleavable linkers

KD Park, R Liu, H Kohn

Index: Park, Ki Duk; Liu, Rihe; Kohn, Harold Chemistry and Biology, 2009 , vol. 16, # 7 p. 763 - 772

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Citation Number: 47

Abstract

Proteomic searches using affinity-based chromatography (eg, biotin-[strept] avidin) have been severely hampered by low protein recovery yields, protein destruction and denaturation, and the release of background proteins from the support. These limitations confound protein identification. A new acylhydrazone-based cleavable linker was developed to permit the efficient isolation of proteins with a traceable tag allowing detection and ...