Synthesis and properties of oligonucleotides containing 8-bromo-2′-deoxyguanosine

C Fàbrega, MJ Macías, R Eritja

Index: Fabrega, Carme; Macias, Maria J.; Eritja, Ramon Nucleosides, Nucleotides and Nucleic Acids, 2001 , vol. 20, # 3 p. 251 - 260

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Citation Number: 19

Abstract

The preparation of oligonucleotides containing 8-bromo-2′-deoxyguanosine is described. Substitution of G by 8-bromoguanine on an alternating CG decamer stabilizes the Z-form in such a way that the B-form was not observed. Melting temperatures showed that duplexes in which 8-bromo-2′-deoxyguanosine paired with natural bases were much less stable.