Journal of the American Chemical Society

Interaction of pyruvate-thiamin diphosphate adducts with pyruvate decarboxylase. Catalysis through" closed" transition states

R Kluger, T Smyth

Index: Kluger, Ronald; Smyth, Timothy Journal of the American Chemical Society, 1981 , vol. 103, # 5 p. 1214 - 1216

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Citation Number: 39

Abstract

3, R=-C-CCHj lactylthiamin diphosphate (2), has eluded synthesis or i~ olation.~ We have now developed syntheses of 2 and its esters. These compounds have been allowed to interact with wheat germ pyruvate decarboxylase apoenzyme. The results of these interactions provide kinetic information about the nature of the catalytic process.