Bioorganic Chemistry

Inhibition of Acetolactate Synthase Isozyme II fromEscherichia coliby a New Azido-Photoaffinity Sulfonylurea

F Ortéga, J Bastide

Index: Ortega, Florence; Bastide, Jean Bioorganic Chemistry, 1997 , vol. 25, # 4 p. 261 - 274

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Citation Number: 6

Abstract

The sulfonylurea herbicides are very potent inhibitors of acetolactate synthase (ALS). These compounds have been reported as “extraneous inhibitors” due to the fact that their inhibition site corresponds to neither the catalytic site nor the regulatory sites of the enzyme. So far, the complexity of the ALS reaction and the reversible binding mode of sulfonylureas have hampered any attempt to locate the inhibitor domain. Toward this goal, a photoactivatable ...